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Use of freeze-cracking in ontogenetic research in Macrostomum lignano (Macrostomida, Rhabditophora)
Willems, M.; Boone, M.; Couvreur, M.; De Mulder, K.; Van Ranst, J.; Artois, T.; Borgonie, G. (2009). Use of freeze-cracking in ontogenetic research in Macrostomum lignano (Macrostomida, Rhabditophora). Dev. Genes Evol. 219(5): 273-279. https://dx.doi.org/10.1007/s00427-009-0284-x
In: Development, Genes and Evolution. Springer: Berlin; Heidelberg. ISSN 0949-944X; e-ISSN 1432-041X
Peer reviewed article  
Available in  Authors 
    Vlaams Instituut voor de Zee: Non-open access 317785 [ request ]
Keywords
    Platyhelminthes [WoRMS]
    Marine/Coastal
Author keywords
    Platyhelminthes; Freeze-cracking; Embryonic development; Eggshell; Immunohistochemistry
Authors  Top 
  • Willems, M.
  • Boone, M.
  • Couvreur, M.
  • De Mulder, K.
  • Van Ranst, J.
  • Artois, T.
  • Borgonie, G.
Abstract
    A method for studying whole mount flatworm embryos based on freeze-cracking of the eggs is described. This method allows successful immunohistological and immunocytological studies of whole mount embryos. It does not require the use of sharpened needles or a microinjection system to puncture the eggshell. Moreover, this method is more practical and less time-consuming than classical puncturing and much cheaper than the use of a microinjection system. The advantages of this method are illustrated by results of several immunolocalisation experiments in the macrostomid flatworm Macrostomum lignano. The optimal procedure and crucial steps for this method are discussed.
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