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Microorganisms (Bacteria, Archaea and phototroph eukaryotes) from Fildes Bay, King George Island, Antarctica
Citation
Moreno-Pino M, De la Iglesia R, Valdivia N, Hendriquez-Castilo C, Galan A, Diez B, Trefault N (2019): Microorganisms (Bacteria, Archaea and phototroph eukaryotes) from Fildes Bay, King George Island, Antarctica. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbes_fildes_bay_antarctica&v=1.1 https://doi.org/10.15468/sr1hmq
Contact:
Moreno-Pino, Mario ;
Trefault, Nicole
Availability: This dataset is licensed under a Creative Commons Attribution 4.0 International License.
Description
Amplicon sequencing dataset (Illumina MiSeq and 454 pyrosequencing) of Bacteria and Archaea (16S ssu rRNA gene) and phototroph eukaryotes (16S chloroplast) from sea water in Fildes Bay, King George Island, Antarctica. more
Surface water samples (5 m depth) were collected with 5 L Niskin bottles from 17 different locations. Each location was assigned to one of the following four categories: Collins Glacier (‘C’ stations), Nelson Glacier (‘N’ stations), Fildes Bay (‘F’ stations) and Inner Bay (‘IB’ stations). Seawater samples (5 L) were prefiltered on board through 100 μm pore mesh and stored in acid-washed carboys and kept on dark until subsampling at the laboratory.
Study Extent: Water samples were taken at Fildes Bay, King George Island, Antarctica, on 7 and 8 February 2012.
Method step description:
Surface water samples (5 m depth) were collected with 5 L Niskin bottles from 17 different locations. Each location was assigned to one of the following four categories: Collins Glacier (‘C’ stations), Nelson Glacier (‘N’ stations), Fildes Bay (‘F’ stations) and Inner Bay (‘IB’ stations). Seawater samples (5 L) were prefiltered on board through 100 μm pore mesh and stored in acid-washed carboys and kept on dark until subsampling at the laboratory.
Study Extent: Water samples were taken at Fildes Bay, King George Island, Antarctica, on 7 and 8 February 2012.
Method step description:
- For photosynthetic eukaryote identification, plastidial 16S rRNA PCR products (in triplicates) were obtained using primer pair PLA491F–OXY1313, purified using Zymoclean kit (Zymo Research) and checked on an Agilent Bioanalzyer DNA1000 chip for the absence of primer dimers, and quantified using a PicoGreen dsDNA quantitation reagent (Invitrogen). Equal amount of purified PCR products were pooled for subsequent 454 pyrosequencing using a Roche GS-FLX Junior.
- For bacterial identification, general 16S rRNA PCR products (in triplicates) were obtained using primers 515Fseq and 806rcbc (Caporaso et al.2011) following conditions from Earth Microbiome Project (EMP) (Gilbert, Jansson and Knight 2014). Illumina primer constructs were obtained from EMP also. Amplicons were quantified using KAPA Library Quantification Kit (KAPA Biosystem) and sequenced using Illumina Miseq following Caporaso et al. (2012) protocol. 12 pM of qPCR quantified amplicons pool were sequenced using a 300 cycles Illumina Miseq kit.
Scope
Keywords:
Marine/Coastal, Dna sequencing, Metadata, Rrna, PSW, Antarctica, South Shetland I., King George I., Archaea, Bacteria
Geographical coverage
Temporal coverage
7 February 2012 - 8 February 2012
Parameter
Molecular data
Contributors
Universidad Mayor, data creator
Moreno-Pino, Mario
Trefault, Nicole
Trefault, Nicole
Pontifical Catholic University of Chile, data creator
De la Iglesia, Rodrigo
Hendriquez-Castilo, Carlos
Diez, Beatriz
Hendriquez-Castilo, Carlos
Diez, Beatriz
Universidad Austral de Chile, data creator
Valdivia, Nelson
Catholic University of the Most Holy Conception, data creator
Galan, Alexander
Related datasets
Published in:
AntOBIS: Antarctic Ocean Biodiversity Information System
(Partly) included in:
RAS: Register of Antarctic Species
Dataset status: Completed
Data type: Metadata
Data origin: Research: field survey
Metadatarecord created: 2019-04-05
Information last updated: 2019-04-10